%0 Electronic Article %A Wang, Wei and Fan, Lijun and Li, Xuewen and Wang, Zirong and Zhou, Jianzhong and Jialengbieke, Buligen and Ren, Jianye and Hu, Xiaodong %I Wiley %D 2022 %D 2022 %G English %@ 1365-2621 %@ 0950-5423 %~ Berufsakademien Sachsen - Bibliothek %T Digestion of pectic polysaccharide from Brassica rapa L. in vitro and its effect on the intestinal microbiota in cyclophosphamide‐treated mice %V 57 %J International Journal of Food Science & Technology %V 57 %N 1 %P 92-100 %U http://dx.doi.org/10.1111/ijfs.15278 %X AbstractIn this study, the digestion of pectic polysaccharide from Brassica rapa L. (BRP) in vitro and its effect on the intestinal microbiota in cyclophosphamide (Cy)‐treated mice were investigated. As a result, the molecular weight (Mw) of BRP was significantly decreased after simulated gastrointestinal digestion, and content of reducing sugars (CR) was significantly increased (P < 0.05), implying that BRP was gradually degraded under gastrointestinal digestion conditions in vitro. In vivo, both 50 or 200 mg kg−1·BW/d of BRP significantly improved SCFAs concentrations that decreased by Cy treatment (P < 0.05). Meanwhile, BRP enriched the alpha‐diversity of intestinal microbiota and altered the microbial composition with an increase of Lactobacillus and a decrease of Clostridium, Ruminococcus, Oscillospira (P < 0.05). Besides, BRP intervention was conducive to the recovery of mouse body weight, thymus and spleen indices, and contributed to improve the intestinal barrier. %Z https://katalog.ba-sachsen.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9pamZzLjE1Mjc4 %U https://katalog.ba-sachsen.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9pamZzLjE1Mjc4