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Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers

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Journal Title: Brazilian Journal of Biology
Authors and Corporations: Nadeem, A., Hussain, S., Fareed, A., Fahim, M., Iqbal, T., Ahmad, Z., Saeedullah, Karim, R., Akbar, A.
In: Brazilian Journal of Biology, 84, 2024
Type of Resource: E-Article
Language: Undetermined
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FapUNIFESP (SciELO)
author_facet Nadeem, A.
Hussain, S.
Fareed, A.
Fahim, M.
Iqbal, T.
Ahmad, Z.
Saeedullah
Karim, R.
Akbar, A.
Nadeem, A.
Hussain, S.
Fareed, A.
Fahim, M.
Iqbal, T.
Ahmad, Z.
Saeedullah
Karim, R.
Akbar, A.
author Nadeem, A.
Hussain, S.
Fareed, A.
Fahim, M.
Iqbal, T.
Ahmad, Z.
Saeedullah
Karim, R.
Akbar, A.
spellingShingle Nadeem, A.
Hussain, S.
Fareed, A.
Fahim, M.
Iqbal, T.
Ahmad, Z.
Saeedullah
Karim, R.
Akbar, A.
Brazilian Journal of Biology
Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
General Agricultural and Biological Sciences
author_sort nadeem, a.
spelling Nadeem, A. Hussain, S. Fareed, A. Fahim, M. Iqbal, T. Ahmad, Z. Saeedullah Karim, R. Akbar, A. 1678-4375 1519-6984 FapUNIFESP (SciELO) General Agricultural and Biological Sciences http://dx.doi.org/10.1590/1519-6984.253147 <jats:p>Abstract Maydis leaf blight, caused by Bipolaris maydis, is an important disease of maize crop in Khyber Pakhtunkhwa (KP) Pakistan. Fifteen isolates of the pathogen, collected across KP, were studied for variability based on phenotypic and molecular markers. Significant variability among the isolates was observed when assessed using phenotypic traits such as radial growth, spore concentration, fungicide sensitivity and virulence. The isolates were classified into six culture groups based on colour, texture and margins of the colony. Conidial morphology was also variable. These were either straight or slightly curved and light to dark brown in colour. Fungicide test showed significant variation in the degree of sensitivity against Carbendazim. Isolate Bm8 exhibited maximum radial growth on carbendazim spiked plates. Conversely, isolate Bm15 showed the lowest radial growth. Variations in virulence pattern of the isolates were evident when a susceptible maize variety Azam was inoculated with spores of B. maydis. Genetic variability amongst the isolates was also estimated by RAPD as well as sequencing of ITS region. The RAPD dendrogram grouped all the isolates into two major clusters. Average genetic distance ranged from 0.6% to 100%, indicating a diverse genetic gap among the isolates. Maximum genetic distance was found between isolates Bm9 and Bm10 as well as Bm2 and Bm8. Conversely, isolates Bm13 and Bm15 were at minimum genetic distance. Phylogenetic dendrogram based on sequencing of ITS region grouped all the isolates into a single major cluster. The clusters in both the dendrogram neither correlate to the geographical distribution nor to the morphological characteristics.</jats:p> Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers Brazilian Journal of Biology
doi_str_mv 10.1590/1519-6984.253147
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title Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_unstemmed Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_full Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_fullStr Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_full_unstemmed Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_short Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_sort genetic variations among the isolates of bipolaris maydis based on phenotypic and molecular markers
topic General Agricultural and Biological Sciences
url http://dx.doi.org/10.1590/1519-6984.253147
publishDate 2024
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description <jats:p>Abstract Maydis leaf blight, caused by Bipolaris maydis, is an important disease of maize crop in Khyber Pakhtunkhwa (KP) Pakistan. Fifteen isolates of the pathogen, collected across KP, were studied for variability based on phenotypic and molecular markers. Significant variability among the isolates was observed when assessed using phenotypic traits such as radial growth, spore concentration, fungicide sensitivity and virulence. The isolates were classified into six culture groups based on colour, texture and margins of the colony. Conidial morphology was also variable. These were either straight or slightly curved and light to dark brown in colour. Fungicide test showed significant variation in the degree of sensitivity against Carbendazim. Isolate Bm8 exhibited maximum radial growth on carbendazim spiked plates. Conversely, isolate Bm15 showed the lowest radial growth. Variations in virulence pattern of the isolates were evident when a susceptible maize variety Azam was inoculated with spores of B. maydis. Genetic variability amongst the isolates was also estimated by RAPD as well as sequencing of ITS region. The RAPD dendrogram grouped all the isolates into two major clusters. Average genetic distance ranged from 0.6% to 100%, indicating a diverse genetic gap among the isolates. Maximum genetic distance was found between isolates Bm9 and Bm10 as well as Bm2 and Bm8. Conversely, isolates Bm13 and Bm15 were at minimum genetic distance. Phylogenetic dendrogram based on sequencing of ITS region grouped all the isolates into a single major cluster. The clusters in both the dendrogram neither correlate to the geographical distribution nor to the morphological characteristics.</jats:p>
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author Nadeem, A., Hussain, S., Fareed, A., Fahim, M., Iqbal, T., Ahmad, Z., Saeedullah, Karim, R., Akbar, A.
author_facet Nadeem, A., Hussain, S., Fareed, A., Fahim, M., Iqbal, T., Ahmad, Z., Saeedullah, Karim, R., Akbar, A., Nadeem, A., Hussain, S., Fareed, A., Fahim, M., Iqbal, T., Ahmad, Z., Saeedullah, Karim, R., Akbar, A.
author_sort nadeem, a.
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container_title Brazilian Journal of Biology
container_volume 84
description <jats:p>Abstract Maydis leaf blight, caused by Bipolaris maydis, is an important disease of maize crop in Khyber Pakhtunkhwa (KP) Pakistan. Fifteen isolates of the pathogen, collected across KP, were studied for variability based on phenotypic and molecular markers. Significant variability among the isolates was observed when assessed using phenotypic traits such as radial growth, spore concentration, fungicide sensitivity and virulence. The isolates were classified into six culture groups based on colour, texture and margins of the colony. Conidial morphology was also variable. These were either straight or slightly curved and light to dark brown in colour. Fungicide test showed significant variation in the degree of sensitivity against Carbendazim. Isolate Bm8 exhibited maximum radial growth on carbendazim spiked plates. Conversely, isolate Bm15 showed the lowest radial growth. Variations in virulence pattern of the isolates were evident when a susceptible maize variety Azam was inoculated with spores of B. maydis. Genetic variability amongst the isolates was also estimated by RAPD as well as sequencing of ITS region. The RAPD dendrogram grouped all the isolates into two major clusters. Average genetic distance ranged from 0.6% to 100%, indicating a diverse genetic gap among the isolates. Maximum genetic distance was found between isolates Bm9 and Bm10 as well as Bm2 and Bm8. Conversely, isolates Bm13 and Bm15 were at minimum genetic distance. Phylogenetic dendrogram based on sequencing of ITS region grouped all the isolates into a single major cluster. The clusters in both the dendrogram neither correlate to the geographical distribution nor to the morphological characteristics.</jats:p>
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spelling Nadeem, A. Hussain, S. Fareed, A. Fahim, M. Iqbal, T. Ahmad, Z. Saeedullah Karim, R. Akbar, A. 1678-4375 1519-6984 FapUNIFESP (SciELO) General Agricultural and Biological Sciences http://dx.doi.org/10.1590/1519-6984.253147 <jats:p>Abstract Maydis leaf blight, caused by Bipolaris maydis, is an important disease of maize crop in Khyber Pakhtunkhwa (KP) Pakistan. Fifteen isolates of the pathogen, collected across KP, were studied for variability based on phenotypic and molecular markers. Significant variability among the isolates was observed when assessed using phenotypic traits such as radial growth, spore concentration, fungicide sensitivity and virulence. The isolates were classified into six culture groups based on colour, texture and margins of the colony. Conidial morphology was also variable. These were either straight or slightly curved and light to dark brown in colour. Fungicide test showed significant variation in the degree of sensitivity against Carbendazim. Isolate Bm8 exhibited maximum radial growth on carbendazim spiked plates. Conversely, isolate Bm15 showed the lowest radial growth. Variations in virulence pattern of the isolates were evident when a susceptible maize variety Azam was inoculated with spores of B. maydis. Genetic variability amongst the isolates was also estimated by RAPD as well as sequencing of ITS region. The RAPD dendrogram grouped all the isolates into two major clusters. Average genetic distance ranged from 0.6% to 100%, indicating a diverse genetic gap among the isolates. Maximum genetic distance was found between isolates Bm9 and Bm10 as well as Bm2 and Bm8. Conversely, isolates Bm13 and Bm15 were at minimum genetic distance. Phylogenetic dendrogram based on sequencing of ITS region grouped all the isolates into a single major cluster. The clusters in both the dendrogram neither correlate to the geographical distribution nor to the morphological characteristics.</jats:p> Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers Brazilian Journal of Biology
spellingShingle Nadeem, A., Hussain, S., Fareed, A., Fahim, M., Iqbal, T., Ahmad, Z., Saeedullah, Karim, R., Akbar, A., Brazilian Journal of Biology, Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers, General Agricultural and Biological Sciences
title Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_full Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_fullStr Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_full_unstemmed Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_short Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
title_sort genetic variations among the isolates of bipolaris maydis based on phenotypic and molecular markers
title_unstemmed Genetic variations among the isolates of Bipolaris Maydis based on phenotypic and molecular markers
topic General Agricultural and Biological Sciences
url http://dx.doi.org/10.1590/1519-6984.253147